It is generally agreed that good gut health is effective against intestinal pathogens, a strategy that has only been made possible through the removal of antibiotic growth promoters in feed. Creating and maintaining a healthy intestinal environment has become essential to dietary programmers. The current study discusses the impact of a diformate-monolaurate agglomerate in the lactation diet of sows on their microbiome and the resulting impact towards their subsequent litters. This inclusion not only provides a healthy gut in sows, but furthermore supports a pork production chain with reduced zoonotic pathogen pressure, thus being an essential part of any biosecurity program on-farm.